Cytotoxic and antigenotoxic activities of phosvitin from egg yolk

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Cytotoxic and antigenotoxic activities of phosvitin from egg yolk.

Egg yolk phosvitin is one of the most phosphorylated proteins in nature, and thus has a strong metal-binding ability. The objective of this study was to evaluate the cytotoxic and antigenotoxic activities of phosvitin in vitro. Using the 3-[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, the cytotoxicity of phosvitin was evaluated in human cancer cell lines of various tiss...

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Egg yolk phosvitin and functional phosphopeptides--review.

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Separation of Phosvitin from Egg Yolk without Using Organic Solvents

The objective of this study was to develop a new method to separate phosvitin from egg yolk without using organic solvents. Phosvitin was extracted from yolk granules using 10% NaCl or 10% (NH4)2SO4 (final concentration) and then treated with heat to precipitate the lipoproteins from the extracted solution. The optimal pH for the phosvitin extraction from yolk granules was determined, and the i...

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Sequential separation of immunoglobulin Y and phosvitin from chicken egg yolk without using organic solvents.

A study was conducted to develop a simple sequential separation protocol to separate phosvitin and IgY from egg yolk without using organic solvents. Egg yolk was diluted with 2 volumes of distilled water (DW), homogenized, and centrifuged. The precipitant was collected and homogenized with 4 volumes of 10% NaCl (wt/vol) in 0.05 N NaOH solution to extract phosvitin. The pH of the homogenate was ...

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Elucidation of Antioxidant Activity of Phosvitin Extracted from Egg Yolk using Ground Meat

Elucidation of Antioxidant Activity of Phosvitin Extracted from Egg Yolk using Ground Meat Samooel Jung, Cheorun Jo, Mingu Kang, Dong Uk Ahn, and Ki Chang Nam* Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Korea Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 305-764, Korea Department of Animal Science, Iowa Sta...

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ژورنال

عنوان ژورنال: Poultry Science

سال: 2014

ISSN: 0032-5791

DOI: 10.3382/ps.2013-03784